Beta-galactosidase is coded by a gene (lac z) in the lac operon of Escherichia coli.
It is a metalloenzyme that splits lactose into glucose and galactose.
It hydrolyzes terminal, non-reducing beta-D-galactose residues in beta-D-galactosides.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:5000-1:20,000Western Blot: 1:1000-1:10,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：1mg
Host：rabbit
Source Antibody：E. coli
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide.
Specificity：Recognizes beta-galactosidase from E. coli showing a 464kD band (tetramer) under non-reducing conditions of SDS-PAGE and 116kD band under reducing conditions.
