The recombinant protein over-expression systems with well-known epitope tags are powerful strategies in checking and purifying the interest proteins, which are expressed in certain vectors containing the particular tagged fragments.
The small tags are used to be cloned along with the target proteins, and the antibodies against those fusion-tags are used to monitor fusion protein expression and purification.
E-Tag (GAPVPYPDPLEPR) is a popular epitope tag incorporated in the amino or carboxy terminus of the target protein.
This antibody can be used to check E- tagged fusion proteins.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 0.05-0.2ug/mlWestern Blot: 0.1-1ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：40ug
Host：rabbit
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.01% thimerosal. Labeled with horseradish peroxidase (HRP).
Specificity：Specifically binds to over-expressed fusion proteins containing E epitope tags.
Isotype：IgG
Calc Applications Abbrev：E WB
Immunogen：E epitope tag peptide GAPVPYPDPLEPR conjugated to KLH
