The recombinant protein over-expression systems with well-known epitope tags are powerful strategies in checking and purifying the interest proteins, which are expressed in certain vectors containing the particular tagged fragments.
The small tags are used to be cloned along with the target proteins, and the antibodies against those fusion-tags are used to monitor fusion protein expression and purification.
E-Tag (GAPVPYPDPLEPR) is a popular epitope tag incorporated in the amino or carboxy terminus of the target protein.
This antibody can be used to check E- tagged fusion proteins.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 0.05-0.2ug/mlWestern Blot: 0.1-1.0ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：40ug
Host：rabbit
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide.
Specificity：Recognizes E Epitope Tag fusion proteins.
Isotype：IgG
Calc Applications Abbrev：E WB
Immunogen：Peptide corresponding to GAPVPYPDPLEPR from E Epitope Tag, conjugated to KLH.
