c-erbB-2 is a membrane-associated oncoprotein having considerable sequence homology to EGFR.
C-erbB-2 is the second member of the EGF receptor tyrosine kinase family of growth factor receptors.
Overexpression of c-erbB-2/HER-2 is reportedly a marker of poor prognosis in node-positive and in defined subsets of node-negative breast cancer.
Elevated levels of c-erbB-2/HER-2 has also been implicated in response to chemotherapy.
Applications:Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000 Immunohistochemistry: 1:50-1:100Immunofluorescence: 1:100-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Specificity：Detects endogenous levels of HER2 only when phosphorylated at tyrosine 1248.
Isotype：IgG
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthesized phosphopeptide derived from human HER2 around the phosphorylation site of tyrosine1248 (P-E-YP-L-G)
