c-erbB-2 is a membrane-associated oncoprotein having considerable sequence homology to EGFR.
C-erbB-2 is the second member of the EGF receptor tyrosine kinase family of growth factor receptors.
Overexpression of c-erbB-2/HER-2 is reportedly a marker of poor prognosis in node-positive and in defined subsets of node-negative breast cancer.
Elevated levels of c-erbB-2/HER-2 has also been implicated in response to chemotherapy.
Applications:Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:100Immunofluorescence: 1:100-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:For long-term storage, aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Specificity：Detects endogenous levels of total HER2 protein.
Isotype：IgG
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthesized non-phosphopeptide derived from human HER2 around the phosphorylation site of tyrosine 1221/1222 (N-L-YP-YP -W)
