Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells.
Disruption of this process has been implicated in a variety of diseases such as cancer.
Bcl-2 is the founding member of a family of over 20 proteins that are critical regulators of apoptosis.
These can be divided into two classes: those that inhibit apoptosis and those that promote cell death.
Bcl-2 is an inner mitochondrial membrane protein that inhibits apoptosis.
It is thought to act by interacting with pro-apoptotic Bcl-2 family members such as Bak and Bad.
Overexpression of Bcl-2 has been linked to human cancers such as B-cell lymphoma and prostate cancer.
Applications:Suitable for use in Immunofluorescence, Immunohistochemistry and Western Blot.
Other applications not tested.
Recommended Dilution:Immunohistochemistry: 1:50-1:100Western Blot: 1:500-1:1000Immunofluorescence: 1:100-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:For long-term storage, aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide and 50% glycerol.
Specificity：Recognizes endogenous levels of total BCL-2 protein.
Isotype：IgG
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthesized non-phosphopeptide derived from human bcl-2 around the phosphorylation site of serine 70 (R-T-S-P-L)
