A number of mutations, identified in the gene encoding the beta-amyloid precursor protein (betaAPP), have been linked to early-onset Familial Alzheimers Disease.
betaAPP is cleaved sequentially by the proteolytic enzymes beta-secretase and gamma-secretase to produce beta-amyloid (Abeta) peptides with the Abeta1-42(43) and the Abeta1-40 forms being the most prevalent.
Secreted Abeta peptides can bind to scavenger receptors and the receptor for advanced glycation endproducts.
Abeta peptides are degraded either via a reuptake mechanism followed by endosomal degradation or by an extracellular insulin-degrading enzyme.
Extracellular accumulation of Abeta leads to formation of aggregates, fibrils, and eventually amyloid deposits called neuritic plaques, a hallmark of Alzheimer’s disease.
beta-Amyloid antibodies and peptides have been developed as tools for elucidating the biology of Alzheimers disease.
Applications:Suitable for use in Immunofluorescence, ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 5-20ug/ml ELISA: 0.05-0.5ug/mlWestern Blot: 0.1-1.0ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：40ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide.
Specificity：Specific to human beta-amyloid 1-40. Do not expect it to react with human beta-amyloid 1-42.
Isotype：IgG
Calc Applications Abbrev：E IF WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to aa32-40 of human beta-Amyloid conjugated to KLH
