Stromal interaction molecule 1 plays a role in mediating Ca(2+) influx following depletion of intracellular Ca(2+) stores.
It acts as Ca(2+) sensor in the endoplasmic reticulum via its EF-hand domain.
Upon Ca(2+) depletion, it translocates from the endoplasmic reticulum to the plasma membrane where it activates the Ca(2+) release-activated Ca(2+) (CRAC) channel subunit.
A band is seen at ~77kD, representing the STIM-1 protein.
Two other non-specific bands of lower MW can also be seen
Optimal dilutions to be determined by the researcher.
Cellular Localization: Cell membrane, ER membrane.
Translocates from ER to cell membrane in response to depletion of intracellular calcium.
Positive Controls: HeLa whole cell lysate
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 30% glycerol, 0.09% sodium azide.
Specificity：Recognizes human STIM1. Species Crossreactivity: 100% homology with human, mouse, rat, bovine, and canine proteins. 79% homology with Xenopus protein.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to a region between aa600 and 685 pf human protein STIM1.
