This gene is a member of the leucine-rich repeat kinase family and encodes a protein with an ankryin repeat region, a leucine-rich repeat (LRR) domain, a kinase domain, a DFG-like motif, a RAS domain, a GTPase domain, a MLK-like domain, and a WD40 domain.
The protein is present largely in the cytoplasm but also associates with the mitochondrial outer membrane.
Mutations in this gene have been associated with Parkinson disease-8.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 2 ug/ml, A band is seen at ~284kD.
There is a band at ~171 and 117kD, as well as several lower molecular weight, non-specific bands.
Immunohistochemistry: 2.5-5.0ug/ml, (Paraffin): 2.5-5.0ug/mlOptimal dilution determined by the researcher.
Positive Controls: Mouse brain lysate
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris-citrate-phosphate, pH 7-8, 0.09% sodium azide.
Specificity：Recognizes with the mouse protein but does not react with the human protein. Detection of murine LRRK2 in mouse brain lysate.Homology: 100% mouse. 87% human.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Mo
Immunogen：A synthetic peptide made to an internal region of the mouse LRRK2 protein sequence (between residues 900-1000). [Swiss-Prot# Q5S006]
