This gene is a member of the leucine-rich repeat kinase family and encodes a protein with an ankryin repeat region, a leucine-rich repeat (LRR) domain, a kinase domain, a DFG-like motif, a RAS domain, a GTPase domain, a MLK-like domain, and a WD40 domain.
The protein is present largely in the cytoplasm but also associates with the mitochondrial outer membrane.
Mutations in this gene have been associated with Parkinson disease-8.
Applications:Suitable for use in ELISA, Immunohistochemistry (Paraffin), Immunoflourescence and Western Blot.
Other applications not tested.
Recommended Dilution:Immunohistochemistry: 1:100 Immunofluorescence: 1:1000 (Mouse CAD cells transfected with human wild-type LRRK-2, immunoprecipitation: 7ul/15 cm plate of SH-SY5Y cells grown to 80% conflue, Western Blot: 1:1(the theoretical molecular weight of LRRK2 is ~286kD.
Other bands have been seen with some lysates, but these bands have been blocked by the control peptide, L4502-21E, suggesting that these bands are degredation products)Optimal dilution determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid.
Specificity：Recognizes human LRRK2. Species Croossreactivity: Bovine. Negative in mouse. There is 87% homology of the immunogen with the rat LRRK2 proteins. Homology: 100% homology with human protein, 94% with mouse and rat protein.
Isotype：IgG
Calc Applications Abbrev：E IF IHC IP WB
Calc Crossreactivity：Hu Mo
Immunogen：A C-terminal synthetic peptide made to the human LRRK2 protein sequence (between residues 2500-2527).
