84-1091-83　［取扱停止］CIC (Capicua, KIAA0306) 100ul　C0030-03B2

特徴

• CIC, a human homolog of Drosophila capicua, encodes a high mobility group box transcription factor and is fused to a double homeodomain gene DUX4 as a result of a recurrent chromosomal translocation.
• It is involved in epidermal growth factor receptor (EGFR) signaling, developmental patterning, and cell fate.
• In humans, CIC may be involved in granule cell lineage, cerebellar development, and medulloblastoma tumorigenesis.
• Recent evidence indicates that CIC is involved in Spinocerebellar Ataxia Type 1 (SCA-1) where it interacts directly with the product of the SCA-1 gene Ataxin-1.
• Applications:Suitable for use in ELISA, Western Blot, Immunoprecipitation.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000 -1:2500.
• A band is seen at ~250kD.
• CIC (~164kD) interacts with Ataxin 1 (~87kD).
• This may explain the band at 250kD (complex)
• Immunoprecipitation: 2-5ug/mg lysateOptimal dilutions to be determined by the researcher.
• Positive Controls:HeLa whole cell lysate
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.