ApplicationsSuitable for use in block receptor-ligand interactions, ELISA, Western Blot, and Immunohistochemistry, and Flow cytometry.
Other applications have not been tested.
Recommended Dilutions:Blockade of receptor-ligand interaction-Approximately 0.3-1ug/ml of this antibody will block 50% of the binding of 100 ng/ml of rmuPAR-1/Fc to immobilized huPA (100il of a 2ug/ml solution was coated in each well) in a functional ELISA assay
ELISA: 0.5-1.0ug/ml The detection limit is ~0.2 ng/well
Western blot-: 0.1-0.2ug/mL.
The detection limit is ~5 ng/lane under non-reducing and reducing conditions
Immunohistochemistry: 5-15ug/ml in cells and tissues.
Flow cytometry: 3-10ug/mL/106 cells for indirect immunofluorescence staining of cells by flow cytometry
Optimal dilutions to be determined by the researcher.
Storage and Stability:Aliquot to avoid repeated freezing and thawing and freeze at -70°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Aliquots are stable for at least 12 months.
仕様
Size：100ug
Host：goat
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography. Endotoxin: < 0.1 EU per 1ug of the antibody as determined by the LAL method.
Form：Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 0.5ml sterile 40-50% glycerol, PBS.
Specificity：Recognizes mouse uPAR-1. In direct ELISAs and western blots, this antibody shows less than1% cross-reactivity with rhuPAR.
