Applications:Suitable for use in Western Blot and ELISA.
Other applications have not been tested.
Recommended Dilutions:Western Blot: 0.1-0.2ug/mL.
The detection limit is~20 ng/lane under non-reducing and reducing conditions
ELISA: coat plate with 100uL/well of the capture antibody, in combination with 100uL/well of thedetection antibody at 200 ng/mL, an ELISA for sample volumes of 100il can beobtained.
To arrive at the optimal dose range for this ELISA, set up a two-folddilution series of the protein standard starting with 2 ng/mL.
In this format, lessthan 0.1% cross-reactivity with rmuPAR and rhuPA-1 is observed
Optimal dilutions to be determined by the researcher.
Storage and Stability:Aliquot to avoid repeated freezing and thawing and freeze at -70°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Aliquots are stable for at least 12 months.
仕様
Size：50ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS and BSA. LAbeled with Bioin. Reconstitute with 0.1% BSA in TBS.
