Integrin Linked Kinase 1 Associated Phosphatase (ILKAP), also called PP2Cdelta, is a serine/threonine phosphatase that is stimulated by Mn 2+ ions but inhibited by Mg 2+ and is insensitive to the phosphatase inhibitor okadaic acid.
It binds to ILK1 and dephosphorylates it, decreasing the stimulation of the GSK3 beta pathway by cell adhesion without affecting Akt signaling.
Transfection studies have shown that ILKAP activity inhibits cell cycle progression from the G1 to S phase.
ILKAP is expressed in many tissues but is highest in skeletal and cardiac muscle.
Its gene is found in a region of chromosome 2 that is frequently deleted in oral cancers.
Applications:Suitable for use in Western blot.
Other applications have not been tested.
Recommended Dilutions:Western Blot: 1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
Reconstitute to nominal volume by adding sterile 40-50% glycerol, aliquot and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Affinity purified on a column derivatized with the recombinant protein and further purified by isolating the IgG fraction.
Form：Supplied as a lyophilized powder in PBS with 5% trehalose. Reconstitute with PBS containing 0.02% NaN3.
Specificity：Recognizes human, mouse and rat ILKAP. detects endogenous human ILKAP at 47 kDa using Western blot. This is higher than the predicted molecular weight of 43 kDa but is consistent with the electrophoretic migration of rhILKAP. By direct ELISA, the antibody is selective for ILKAP over PP2C alpha, beta and gamma by 25-, 16-, and 92-fold, respectively.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：E. coli-derived recombinant human rhILKAP (aa 1- 392).
