Applications:Suitable for use in Western Blot and Flow Cytometry.
Other applications have not been tested.
Recommended Dilution:Western Blot: 0.1ug/ml using recombinant human Granulysin
Flow Cytometry: 2.5ug per 1 million cels.
Total reaction volume should not exceed 200ul.
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size:50ug
Host:goat
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a powder lyophilized from a solution containing PBS and BSA. Labeled with Biotin. Reconstitute to a concentration of 0.2mg/ml with 250ul sterile PBS.
Specificity:Recognizes human Granulysin. Cross-reactivity: <1% with rhNKG-2D.
Isotype:IgG
Calc Applications Abbrev:FC WB
Calc Crossreactivity:Hu
Immunogen:NS0-derived recombinant protein corresponding to Arg23 thru Leu145 of human Granulysin, UniProt accession #P22749.