84-1091-62　Dentin Matrix Protein 1 (DMP1) 100ug　D8075-51

特徴

• DMP-1 (dentin matrix protein 1) is an acidic, 100-160kD member of the phosphophoryn family of extracellular matrix proteins.
• Mouse DMP-1 is found both intra- and extracellularly, where phosphorylated DMP-1 participates in bone mineralization.
• Secreted mature mouse DMP-1 is a 487 amino acid (aa) proteoglycan.
• It contains multiple serine, glutamic acid, and aspartic acid residues, a GAG attachment site (aa71-72) and an RGD motif (aa350-352).
• Multiple cleavage sites exist between aa205-253, creating multiple fragments ~37kD and 57kD in size.
• Applications:Suitable for use in Western Blot, Immunohistochemistry and ELISA.
• Other applications have not been tested.
• Recommended Dilutions:Western Blot: 0.1-0.2ug/ml The detection limit is ~1ng/lane and 5ng/lane under non-reducing and reducing conditions, respectively
• Immunohistochemistry: 10ug/ml detects DMP1 in E15.5 mouse embryos.
• Fix cells with PBS, 4% paraformaldehyde for 20 minutes at RT and block with PBS, 10% normal donkey serum, 0.1% Triton X-100, 1% BSA for 45 minutes at RT.
• Incubate cells with D8075-51 for 3 hours at RT followed by donkey anti-sheep lgG at RT for 1 hour.
• Between each step, wash cells with PBS, 0.1% BSA.
• Direct ELISA: 0.5-1.0ug/ml The detection limit is ~0.1ng/well.
• Optimal dilutions to be determined by the researcher.
• Hybridoma:NSO myeloma cells with spleen cells from Balb/c mice.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.