DPP10 (dipeptidyl peptidase 10; also DPPY, DPRP3 and DPL2) is a 91-97kD member of the peptidase S9B family.
It is a type II transmembrane glycoprotein principally expressed in neurons and T cells.
Although it is a peptidase member, it has no catalytic activity.
Instead, it assists in the membrane trafficking and functioning of Kv4 K + channels.
Human DPP10 is 796 amino acids (aa) in length.
It contains a 34 aa cytoplasmic N-terminus and a 741 aa extracellular domain (ECD) (aa 56-796).
Three potential isoforms exist, all involving aa substitutions limited to the first 20 amino acids.
Produced in sheep immunized with purified, Sf 21-derived, recombinant human DPP10 isoform 1 extracellular domain (rhDPP10; aa 56-796; Accession # NP_065919, with a Pro340Ala substitution).
Human DPP10 specific IgG was purified by human DPP10 affinity chromatography and then biotinylated.
Applications:Suitable for use in Western blot.
Other applications have not been tested.
Recommended Dilutions:Western blot: 0.
-0.2ug/ml with the The detection limit is ~2ng/lane under non-reducing and reducing conditions.
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
Reconstitute to nominal volume by adding sterile 40-50% glycerol, aliquot and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：50ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in PBS with BSA. Reconstitute with 1ml sterile 0.1% BSA in TBS to a concentration of 0.05mg/ml.
Specificity：Recognizes human DPP10 extracellular domain. The ECD of human DPP10 shares 89% aa sequence identity with mouse DPP10 ECD. < 5% cross-reactivity with rhDDP4 and rhDPP6.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Sf 21-derived recombinant human rhDPP10 extracellular domain isoform1.
