Clock is an essential regulator of circadian rhythms.
Clock is a basic helix-loop-helix-PAS transcription factor that heterodimerizes with BMAL1.
Together these transcription factors bind to E box elements (CACGTG) within the promoter of circadian genes (period1 and 2) stimulating their transcription.
BMAL dimerization is necessary for Clock’s nuclear translocation, E box dependent transcription, phosphorylation, and degradation.
Applications:Suitable for use in ELISA, Western Blotting.
Other applications not tested.
Recommended Dilution:Western Blotting: 0.5ug/mLOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in PBS, 0.02% trehalose. Reconstitute with 1ml sterile 40-50% glycerol, PBS.
Specificity：Recognizes human Clock.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：E. coli-derived recombinant human Clock.
