84-1091-57　CEACAM 3 (Carcinoembryonic antigen-related cell adhesion molecule 3, CD66d, CGM1) 50ug　C2589-68B

特徴

• Carcinoembryonic antigen-related cell adhesion molecule 3; also CD66d and CGM1) is a 31-34kD member of the CEACAM subfamily of the CEA family of proteins.
• It is expressed by neutrophils and serves as a microbial receptor for a wide range of microorganisms.
• Mature human CEACAM-3 is a 218 amino acid (aa) type I transmembrane glycoprotein.
• Its extracellular domain (aa 35-155) shows one V-type Ig-like domain aa 35-142).
• There are two alternate splice forms.
• One shows a 40 aa substitution for the C-terminal 116 amino acids (aa 137-252), while a second shows a 31 aa substitution for the C-terminal 71 amino acids.
• No definitive rodent CEACAM-3 has been reported.
• Applications:Suitable for use in Western blot.
• Other applications have not been tested.
• Recommended Dilutions:Western blot: 0.
• -0.2ug/ml with the The detection limit is ~1ng/lane under non-reducing and reducing conditions.
• Optimal dilutions should be determined by each laboratory for each application.
• Hybridoma:Produced in sheep immunized with purified, NS0-derived, recombinant human rhCEACAM-3; aa 33-155; Accession # P40198).
• Human CEACAM-3 specific IgG was purified by human CEACAM-3 affinity chromatography and then biotinylated.
• Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
• Reconstitute to nominal volume by adding sterile 40-50% glycerol, aliquot and store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.