CD31, also known as PECAM-1, is a 130kD type I transmembrane glycoprotein adhesion molecule in the Ig superfamily.
Interaction of CD31 on endothelial cells with CD31 on monocytes, neutrophils and lymphocyte subsets is required for transendothelial migration.
In platelets, CD31 is necessary for limiting thrombus formation.
Porcine CD31 extracellular domain contains six C2-type Ig-like domains and shows 74%, 73% and 70% amino acid (aa) identity with bovine, canine and human CD31, respectively.
The 572aa ectodomain may be shed by metalloproteinase cleavage.
Applications:Sutable for use in Western Blot and Flow Cytometry.
Other applications have not been tested.
Recommended Dilutions:Western Blot: 0.1ug/ml using recombinant mouse CD31/PECAM‑1 Flow Cytometry: 2.5ug labels 10e6 cells using mouse splenocytesOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：50ug
Host：goat
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, BSA. Reconstitute with 250ul sterile PBS. Labeled with Biotin.
Specificity：Recognizes mouse CD31. Species sequence homology: recombinant human CD31 and recombinant porcine CD31: 10%.
Isotype：IgG
Calc Applications Abbrev：FC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Glu18-Lys590 from mouse CD31, expressed in NSO cells.
