Casein kinase 2 (CK2) is a ubiquitous, tetrameric serine/threonine kinase comprised of two catalytic subunits (CK2a and/or CK2a‘) and two identical regulatory subunits (CK2b).
CK2 has been implicated in numerous cellular processes, including signal transduction, transcription, translation, replication, and metabolic pathways.
CK2b undergoes phosphorylation at Ser-2, Ser-3 and Ser-209.
CK2b also plays dual roles in the regulation of CK2 acivity.
The C-terminal domain is responsible for stable interactions with the catalytic subunit and increased catalytic activity following tetramer formation, while the N-terminal domain exerts negative regulation on the catalytic activity of CK2.
CK2 is known to phosphorylate more than 300 different substrates.
Phosphorylation of cell-cycle proteins such as p53, p34cdc2, p27KIP1, and MDM-2 account for the ability of CK2 to induce proliferation, while the phosphorylation of HS1, Bid, and Max account for its antiapoptotic role.
Applications:Suitable for use in Western Blot and Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1ug/mlImmunocytochemistry: 5-15ug/mlOptimal dilution determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Specificity：Recognizes human CK2β. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：IC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant protein corresponding to Met1-His153 of human CK2β expressed in E. coli.
