Casein Kinase 1 (CK1) represents a group of serine/threonine protein kinases that are present in all eukaryotic organisms.
Human CK1 isoforms (a, γ1, γ2, γ3, δ and ε) act as monomeric constitutively active enzymes that phosphorylate key regulatory proteins involved in the control of cell differentiation, proliferation, chromosome segregation, and circadian rhythms.
CK1 family members share a highly conserved kinase domain but differ in their variable N- and C-terminal domains.
CK1δ phosphorylates p53 as well as MDM2 and seems to play a principal role in coordinating the effects of these two key regulator proteins on cell growth and genome integrity
Preparation: Sheep antibodies were raised against purified, E.
coli-derived recombinant human CK1δ (rhCK1δ; aa 316-415).
Polyclonal antibody was affinity-purified on a column derivatized with the recombinant protein and further purified by isolating the IgG fraction.
Applications:Suitable for use in Western Blot.
Other applications have not been tested.
Recommended Dilutions:Western blot: 1ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Reconstitute to nominal volume by adding sterile buffer.
Aliquot and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 1ml sterile PBS.
Specificity：Recognizes human mouse, and rat Casein Kinase 1 delta. Does not cross-react with rhCK1α or rhCK1γ; reactivity with rhCK1ε is unknown.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：E. coli-derived recombinant human CK1δ, Arg316-Arg415
