The calcium/calmodulin (CaM) kinase signaling cascade includes the upstream kinases CaM kinase kinase a (CaMKKa; also known as CaMKK1) and CaMKKb (CaMKK2), and their substrates, the output kinases CaMKI and CaMKIV.
Largely by activation of the transcription factor CREB, the CaM kinase cascade is implicated in neuronal development and long-term memory formation.
In addition, CaMKKa is known to modulate skeletal muscle glucose uptake, and is upregulated during retinoic acid-induced neutrophil maturation.
Application:Suitable for use in Western blot.
Other applications have not been tested.
Recommended Dilutions:Western Blot: 1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
Reconstitute to nominal volume by adding sterile 40-50% glycerol, aliquot and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Affinity-purified on a column derivatized with the recombinant protein and further purified by isolating the IgG fraction.
Form：Supplied as a lyophilized powder in PBS with 5% trehalose. Reconstitute with PBS containing 0.02% NaN3.
Specificity：Recognizes endogenous human and rat CaMKK alpha. No cross-reactivity with recombinant human CaMKK beta.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Rt
Immunogen：E. coli-derived recombinant human rhCaMKKα (aa 1-132).
