Beta-IG-H3 (TGFBI/RGD-CAP/Kerato-epithelin) is a 683aa secretory protein induced by TGF-b that plays a role in cell adhesion, differentiation, and apoptosis (1-4).
Beta-IG-H3 contains an internal cysteine-rich EMI domain followed by four fasciclin-1 domains and a carboxyl terminal RGD domain (1,2).
It contributes to cell adhesion through interactions with integrins as well as a number of extracellular matrix (ECM) proteins including collagen, fibronectin, and laminin (5-7).
ECM Beta-IG-H3 is found in a wide variety of tissues (8-12).
Mutations in the Beta-IG-H3 gene as well as elevated protein levels are most notably associated with corneal dystrophies (13).
Applications:Suitable for use in Western Blot, Immunoprecipitation, Immunofluorescence.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Immunoprecipitation: 1:50Immunofluorescence (IF-IC): 1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by peptide affinity chromatography.
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Specificity：Recognizes endogenous levels of total human Beta-IG-H3 protein.
Isotype：IgG
Calc Applications Abbrev：IF IP WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to residues near the C-terminus of human Beta-IG-H3. Species Sequence Homology: monkey (100%)
