The Silent Information Regulator (SIR2) family of genes is a highly conserved group of genes that encode nicotinamide adenine dinucleotide (NAD)-dependent protein deacetylases, also known as Class III histone deacetylases.
The first discovered and best characterized of these genes is Saccharomyces cerevisiae Sir2, which is involved in silencing of mating type loci, telomere maintenance, DNA damage response and cell aging.
SirT6, a mammalian homolog of Sir2, is a nuclear, chromatin-associated protein that promotes the normal maintenance of genome integrity mediated by the base excision repair (BER) pathway.
The BER pathway repairs single-stranded DNA lesions that arise spontaneously from endogenous alkylation, oxidation and deamination events.
SirT6 deficient mice show increased sensitivity to DNA-damaging agents, including the alkylating agents MMS and H2O2.
In addition, these mice show genome instability with increased frequency of fragmented chromosomes, detached centromeres and gaps.
SirT6 may regulate the BER pathway by deacetylating DNA Pol-beta or other core components of the pathway.
Applications:Suitable for use in Immunofluorescence, Western Blot and Immunoprecipitation.
Other applications not tested.
Recommended Dilution:Immunofluorescence: (IF-IC) 1:100Western Blot: 1:1000Immunoprecipitation: 1:50Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by peptide affinity chromatography.
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Specificity：Recognizes endogenous levels of human total SirT6 protein at 42kD. This antibody does not cross-react with other sirtuin proteins.
Isotype：IgG
Calc Applications Abbrev：IC IF IP WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to the C-terminus of the human SirT6 protein.
