RalA and RalB are members of the Ras family of small GTPases and are highly homologous in protein sequence.
The functions of RalA and RalB are distinct yet overlapping.
By binding to various effector proteins, RalA and RalB serve as important GTP sensors for exocytosis and membrane trafficking (1–3).
RalA is required for Ras-related tumorigenesis (4) and RalB is important for tumor survival (5).
In addition to tumor formation, Ral proteins also play a role in cancer cell migration and metastatic tumor invasion (6,7).
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000, incubate membrane with diluted antibody in 5% nonfat dry milk, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight.
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by peptide affinity chromatography.
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Specificity：Recognizes endogenous levels of total human RalB protein. Might also react with overexpressed RalA protein. Species Crossreactivity: monkey
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mk
Immunogen：Synthetic peptide corresponding to residues surrounding Glu142 of human RalB protein.
