84-1091-35　Pyruvate Dehydrogenase 100ul　P9544-45

特徴

• Pyruvate dehydrogenase complex catalyzes the conversion of pyruvate and CoA into acetyl-CoA and CO 2 in the presence of NAD+ (1).
• Acetyl-CoA then goes into citric acid cycle to react with oxaloacetate to form citrate.
• Acetyl-CoA is also used for fatty acid and cholesterol biosynthesis (1).
• The reaction of oxidative decarboxylation of pyruvate therefore serves as a critical link between glycolysis and citric acid cycle and lipid metabolism (1).
• In mammalian cells, the pyruvate dehydrogenase complex is located in the mitochondrial matrix (1).
• This complex is comprised of three enzymes: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and dihydrolipoamide dehydrogenase (E3).
• Pyruvate dehydrogenase (E1) consists of two types of subunits: alpha and beta.
• This enzyme catalyzes the removal of one carbon as CO 2 from pyruvate.
• Mutations in the alpha subunits of pyruvate dehydrogenase (E1) lead to congenital defects that are usually associated with lactic acidosis, neurodegeneration and early death (2).
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.