84-1091-30　Parvin, alpha 100ul　P3113-60

特徴

• The extracellular matrix (ECM) is a complex structure of secreted macromolecules surrounding mammalian organs and tissues.
• Controlled interactions between cells and the ECM are important in proliferation, migration, survival, polarity and differentiation.
• Cells contact the ECM primarily through heterodimeric integral membrane proteins called integrins.
• Integrins connect the ECM to the cytoskeleton, and therefore the cell signaling machinery, through protein complexes called focal adhesions (1).
• The ILK/PINCH/Parvin (IPP) complex is composed of three highly conserved proteins recruited to sites of ECM contact as pre-assembled structures.
• The IPP acts at the interface of the integrin/actin connection to regulate formation of focal adhesions and integrin signaling.
• All three proteins contain multiple protein binding domains allowing them to function as adaptor proteins in the formation of focal adhesions.
• ILK (integrin-linked kinase) also has a catalytic (protein Ser/Thr kinase) domain, but may or may not function as a kinase in vivo.
• Roles for IPP proteins outside of the IPP complex have been proposed, including regulation of gene expression (2,3).
• The parvin family consists of 3 members, alpha-parvin/actopaxin, beta-parvin/affixin and gamma-parvin.
• alpha-parvin and beta-parvin are expressed ubiquitously, while expression of gamma-parvin is restricted to haematopoietic cells (4).
• alpha-parvin binds to F-actin both directly and via interaction with the focal adhesion protein paxillin (5).
• alpha-parvin regulates cell spreading and motility through interactions with the cofilin kinase TESK1 (6), and with the GTPase activating protein CdGAP (7).
• Phosphorylation of alpha-parvin during mitosis may have a role in the regulation of actin dynamics during the cell cycle (8).
• Applications:Suitable for use in Immunofluorescence and Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight.
• Immunofluorescence (IF-IC): 1:400Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.