The Na,K-ATPase is an integral membrane heterodimer belonging to the P-type ATPase family.
This ion channel uses the energy derived from ATP hydrolysis to maintain membrane potential by driving sodium export and potassium import across the plasma membrane against their electrochemical gradients.
It is composed of a catalytic alpha subunit and a beta subunit (1).
Several phosphorylation sites have been identified for the alpha subunit.
Tyr10 is phosphorylated by an as yet undetermined kinase (2), Ser16 and Ser23 are phosphorylated by PKC, and Ser943 is phosphorylated by PKA (3-5).
All of these sites have been implicated in the regulation of enzyme activity in response to hormones and neurotransmitters, altering trafficking and kinetic properties of Na,K-ATPase.
Altered phosphorylation in response to angiotensin II stimulates activity in rat proximal tubule (6).
Na,K-ATPase is also involved in other signal transduction pathways.
Insulin regulates its localization in differentiated primary human skeletal muscle cells, and this regulation is dependent on ERK1/2 phosphorylation of the alpha subunit (7).
Na,K-ATPase and Src form a signaling receptor complex that affects regulation of Src kinase activity and, subsequently, its downstream effectors (8,9).
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:For long-term storage, aliquot and store at -20°C.
Aliquots are stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Specificity：Recognizes endogenous levels of rat Na,KATPase alpha1 only when phosphorylated at Ser16. The residue number, Ser16, is based on the sequence of the immature form of the protein, corresponding to Ser11 of the mature cleaved form.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Rt
Immunogen：Synthetic phosphopeptide corresponding to residues surrounding Ser16 of rat Na,K-ATPase a1. Species sequence homology: mouse, bovine and porcine (100%)
