84-1091-20　IKK epsilon (IKKe) 100ul　I3000-61B

特徴

• The NF-kB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IKB proteins (1-3).
• Most agents that activate NF-KB do so through a common pathway based on phosphorylation-induced, proteasome-mediated degradation of IKB (3-7).
• The key regulatory step in this pathway involves activation of a high molecular weight IKB kinase (IKK) complex, whose catalysis is generally carried out by three tightly associated IKK subunits.
• IKK alpha and IKK beta serve as the catalytic subunits of the kinase.
• IKK gamma serves as the regulatory subunit (8,9).
• Activation of IKK depends on phosphorylation; Ser177 and Ser181 in the activation loop of IKK beta (176 and 180 in IKK alpha) are the specific sites whose phosphorylation causes conformational changes resulting in kinase activation (10-13).
• Recently, two homologs of IKK alpha and IKK beta have been described, called IKK epsilon (also known as IKK-i) and TBK-1 (also known as T2K or NAK), and activation of either of these kinases results in NF-kB activation.
• IKK epsilon contains the kinase domain in its amino terminus, which shares 30% identity to that of IKK alpha or IKK beta.
• IKK epsilon is expressed mainly in immune cells, and may play a special role in the immune response (14-18).
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for at least 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.