Translation is the process where amino acid residues are assembled into polypeptides on ribosomes.
This process is generally divided into three stages: initiation, elongation and termination.
During elongation, mRNA and tRNA pair at the two active sites (A and P sites) on the ribosome.
A variety of eukaryotic elongation factors (eEFs) are involved in this process in mammalian cells.
eEF1A, also called elongation factor Tu (EF-Tu), binds GTP and then interacts with amino acyl-tRNAs and promotes the recruitment of amino acyl-tRNAs to the A-site of the ribosome.
After GTP hydrolysis, GDP-eEF1A leaves the ribosome and is later converted back to the GTP-eEF1A by eEF1B.
Studies have shown that eEF1A is phosphorylated under certain conditions, indicating that its activity is regulated at the post-translational level.
Applications:Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunocytochemistry.
Other applications not tested.
Recommended Dilutions:Immunofluorescence (IF-IC): 1:25Western Blot: 1:1000; Band at ~50kD
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by peptide affinity chromatography.
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Specificity：Recognizes endogenous levels of total human eEF1A protein. Species Crossreactivity: mouse, rat and monkey.
Isotype：IgG
Calc Applications Abbrev：IF WB
Calc Crossreactivity：Hu Mk Mo Rt
Immunogen：Synthetic peptide corresponding to a sequence at the C-terminal end of human eEF1A. Species Sequence Homology: Xenopus.
