Notch signaling is activated upon engagement of the Notch receptor with its ligands, the DSL (Delta, Serrate, Lag2) proteins of single-pass type I membrane proteins.
The DSL proteins contain multiple EGF-like repeats and a DSL domain that is required for binding to Notch (1,2).
Five DSL proteins have been identified in mammals: Jagged1, Jagged2, Delta-like (DLL) 1, 3 and 4 (3).
Ligand binding to the Notch receptor results in two sequential proteolytic cleavages of the receptor by the ADAM protease and the gamme-secretase complex.
The intracellular domain of Notch is released and then translocates to the nucleus where it activates transcription.
Notch ligands may also be processed in a way similiar to Notch, suggesting a bi-directional signaling through receptor-ligand interactions (4-6).
Applications:Suitable for use in ELISA, Western Blot and Immunoprecipitation.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Immunoprecipitation: 1:50Optimal dilutions to be determined by the researcher.
Storage and Stability:For long-term storage, aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity Chromatography.
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol.
Specificity：Recognizes transfected levels of human DLL1 at 82kD. Species Crossreactivity: rat. It does not recognize transfected levels of rat DLL3 or human DLL4.
Isotype：IgG
Calc Applications Abbrev：E IP WB
Calc Crossreactivity：Hu Rt
Immunogen：Synthetic peptide (KLH-coupled) corresponding to a region surrounding residue Ala627 of human DLL1.
