BAP31 (B-cell receptor-association protein 31) is a transmembrane protein associated with the endplasmic reticulum (ER) and ER-Golgi intermediates and has been implicated in protein trafficking and apoptosis (1,2).
During apoptosis Bap31 is cleaved by caspase-8 at two carboxy-terminal sites which can then direct apoptotic signals between the ER and mitochondria (2-4).
Association of BAP31 with the anti-apoptotic proteins Bcl-2 or Bcl-xL could function to regulate this ER-mitochondrial pathway (2,5).
Several studies have shown that BAP31 can control the trafficking of select proteins between the ER and Golgi apparatus and can affect the transport of proteins to the cell surface (6-10).
Applications:Suitable for use in Western Blot and Immunoprecipitation.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Immunoprecipitation: 1:25Optimal dilutions to be determined by the researcher.
Storage and Stability:For long-term storage, aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by peptide affinity chromatography.
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Specificity：Detects endogenous levels of total human BAP31 protein. Species Crossreactivity: mouse, monkey and rat
Isotype：IgG
Calc Applications Abbrev：IP WB
Calc Crossreactivity：Hu Mk Mo Rt
Immunogen：Synthetic peptide corresponding to a region surrounding Arg71 of BAP31.
