This gene is highly similar to the v-src gene of Rous sarcoma virus.
This proto-oncogene may play a role in the regulation of embryonic development and cell growth.
The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase.
Mutations in this gene could be involved in the malignant progression of colon cancer.
Two transcript variants encoding the same protein have been found for this gene.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000 detected Src phosphorylated on residue Tyr215 in lysate from A431 cells stimulated with pervanadate.
Optimal dilutions to be determined by the researcher.
Molecular Weight:~60kD
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 1mg/ml BSA, 0.05% sodium azide, 50% glycerol.
Specificity：Recognizes human phospho-Src (Tyr215). This peptide sequence is somewhat conserved in other Src family kinases, such as fyn, csk, hck, and yes, and may cross-react when phosphorylated in their corresponding regions.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：KLH conjugated peptide corresponding to amino acid region encompassing the phosphorylated Tyr215 residue in human c-Src.
