Paxillin, a focal adhesion protein, is involved in focal adhesion formation during cell adhesion and migration.
Paxillin contains LD motifs, LIM domains, and SH3-/SH2-binding domains that participate in a variety of protein-protein interactions with kinases, GTPase- activating proteins, and cytoskeletal proteins.
Phosphorylation of paxillin occurs at both tyrosine and serine sites.
Serine phosphorylation of paxillin occurs in response to growth- factor activation and fibronectins.
Both JNK1 and cdc2 kinases can phosphorylate serine 178 in paxillin.
The mutant form of paxillin (S178A) decreases the migration of keratocytes and epithelial cells.
Thus, phosphorylation paxillin at serine 178 may be important during cell migration.
Applications:Suitable for use in Western Blot and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500ELISA: 1:2000Optimal dilution to be determined by researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 1mg/ml BSA, 0.05% sodium azide, 50% glycerol.
Specificity：Recognizes a 68kD protein corresponding to the molecular weight of phosphorylated paxillin on SDS-PAGE immunoblots of EGF treated A431 cells, but not in A431 control cells. Similar results were seen in calyculin A treated human A431 and aortic endothelial cells. Epitope: phosphoserine 178. Species Cross Reactivity: Human. This human sequence is highly conserved in rat and mouse paxillin.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide (coupled to KLH) corresponding to amino acid residues around serine 178 of human paxillin. This human sequence is highly conserved in rat and mouse paxillin.
