AS160 is an 160kD Akt substrate that plays a critical role in regulating insulin-stimulated exocytosis of glucose transporter GLUT4.
AS160 contains a Rab GTPase-activating protein domain (GAP), suggesting its potential role in membrane trafficking.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000-1:5000 detected AS160 (Rab-GAP) in RIPA lysates from 3T3/L1 cells differentiated into adipocytes by MDI treatment.
Also detects AS160 (Rab-GAP) in HEK293 lysates
Optimal dilutions to be determined by the researcher.
Positive Control: MDI-treated 3T3/L1 cell lysates
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：rat
Grade：Serum
Purity：Serum.
Form：Supplied as a liquid in 0.05% sodium azide, 30% glycerol.
Specificity：Recognizes rat AS160 (Rab-GAP). Species Crossreactivity: human and mouse
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo
Immunogen：KLH-conjugated, synthetic peptide corresponding to aa1178-1189 (C-PTNDKAKAGNKP) of rat Akt substrate of 160kD (AS160) (Rab-GAP) with a N-terminal cysteine for conjugation purposes. Species Sequence Homology: rat and canine.
