Post-translational modifications of proteins play critical roles in the regulation and function of many known biological processes.
Proteins can be post-translationally modified in many different ways, and a common posttranscriptional modification of Lysine involves methylation.
Lysine can be methylated once, twice or three times by lysine methyltransferases.
The transfer of methyl groups from S-adenosyl methionine to histones is catalyzed by enzymes known as histone methyltransferases.
Histones which are methylated on certain residues can act epigenetically to repress or activate gene expression.
The transcriptional repressor SUV39H1 can encode novel enzymes which selectively methylate histone H3 at lysine 9.
SUV39H1 places a methyl marker on histone H3, which is then recognized by HP1 through its chromo domain.
This model may also explain the stable inheritance of the heterochromatic state.
Some studies have also speculated a stimulatory role for transcription by methylated histone lyside 4 due to its presence at active transcription sites.
Applications:Suitabale for use in Western Blot and ELISA.
Other applications not tested.
Recommended Dilutions:Western Blot: 0.2-0.5ug/ml was sufficient for detection of the methylated histone by Western Blot analysis using melanoma cells in TBSt
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
Labeled with horseradish peroxidase (HRP).
Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
HRP conjugates are sensitive to light.
仕様
Size：400ul
Host：rabbit
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.0, 50% glycerol. Labeled with horseradish peroxidase (HRP).
Specificity：Recognizes proteins containing methylated lysine residues in SDS-PAGE immunoblots. Species Crossreactivity: multi-species.
