The p33ING1 protein encodes a 33-kD, 294-amino acid protein that displays the characteristics of a tumor suppressor gene.
The p33ING1 protein is a regulator of cell cycle, senescence, and apoptosis.
It can mediate growth arrest, anchor dependent growth and influence chemosensitivity (1-4).
It also has a proposed role as a growth regulator, which is consistent with its location in the nucleus (5).
It has been demonstrated that the ING1 gene has three exons and that four mRNA variants are transcribed from three different promoter regions (p33 ING1, p24 ING1, p27 ING1, p47 IG1) (6, 7).
ING1 can possibly influence the above biological functions depending on which variant is expressed (1-4).
Applications:Suitable for use in Western Blot, ELISA and Immunofluorescence.
Other applications not tested.
Recommended Dilution:Western Blot: 0.25mg/ml was sufficient for detection in lysates prepared from human melanoma cell lines by Western Blot analysis
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：400ul
Host：rabbit
Source Antibody：mouse
Grade：Serum
Purity：Serum
Form：Supplied as a liquid in TBS, 0.09% sodium azide.
Specificity：Detects a ~33kD protein, corresponding to the apparent molecular mass of p33 ING1 on SDS-PAGE immunoblots. Species Crossreactivity: Human and Mouse. Not crossreactive with any other ING family.
