Interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8) is a proinflammatory cytokine produced by activated T cells.
This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases.
This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO).
High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis.
Interleukin-17 (IL-17) and its cognate receptor, IL- 17R, are examples of cytokine receptor pairs.
IL-17 protein exhibits a striking degree of homology with the HSV13 protein which mimics its function.
Applications:Suitable for use in Neutralization, Western Blot, Immunocytochemistry, Flow Cytometry, Direct ELISA and Immunoprecipitation.
Other applications not tested.
Recommended Dilutions:Neutralization: The ND50 is 0.02-0.12ug/ml in the presence of 15ng/ml of rhIL-17, using theNIIH/3T3 mouse embryonic fiboblastsImmunocytochemistry: 5-15ug/ml using immersion fixed human peripheral blood mononuclear cells treated with PHAWestern Blot: 1ug/ml using human primary naive CD4+ T cells and human primary differentiated Th17 cellsFlow Cytometry: 2.5ug to label 1x10e6 cells using human peripheral blood mononuclear cells treated with PHA and Ca2+ ionomycin, fixed with paraformaldehyde and permeabilized with saponinImmunoprecipitation: 3ug/100ug cell lysate using human primary differentiated Th17 cellsOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography. Endotoxin: ≤0.1EU/ug
Form：Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.
Specificity：Recognizes human IL-17. Species Crossreactivity: rcIL-17; ~60%, rhIL-17F and rmIL-17; ~10%, rhIL-17B, rhIL;-17C, rhIL-17D, rhIL-17E; <1%
Isotype：IgG
Calc Applications Abbrev：E FC IC IP N WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to aa20-155 from human IL-17 expressed in E. coli (Q16552)
