Green fluorescent protein (GFP), a 27kD protein derived from the jellyfish Aequorea victoria, emits green light (emission peak at a wavelenth of 509nm) when excited by blue light (excitation peak at a wavelenth of 395nm).
Green Fluorescent Protein (GFP) has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells.
GFP fluorescence is stable under fixation conditions and suitable for a variety of applications.
GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining.
Other applications of GFP include assessment of protein-protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols.
GFP is used to measure single cell metastasis and successful proliferation of stem cells.
In these ways, GFP technology has contributed to a greater understanding of cellular biology and biochemistry.
Applications:Suitable for use in Immunocytochemistry and Western Blot.
using colorimetric detection (Peroxidase/TMB), with higher dilution possible using more sensitive detection methods
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography. >99% by SDS-PAGE & HPLC
Form：Supplied as a liquid in TBS, pH 7.2, , 0.01% sodium azide.
Specificity：Recognizes GFP and GFP fusion proteins from both native and recombinant sources. This anti-GFP recognizes all variants of A. victoria GFP induced in transformed organisms, including all vertebrate species tested.
Isotype：IgG
Calc Applications Abbrev：E WB
Immunogen：Highly-purified native GFP from Aequorea victoria.
