Spermidine/spermine-N1-acetyltransferase (SSAT1) is a short-lived polyamine catabolic enzyme which catalyzes the acetylation of polyamines.
This highly regulated enzyme allows a fine attenuation of the intracellular concentration of polyamines.
Induction of SSAT1 plays an important role in polyamine homoeostasis, since the N1-acetylated polyamines can be excreted or oxidized by acetylpolyamine oxidase.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot (transfected lysates): 2ug/ml (ECL)Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:100ul
Host:rabbit
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid in Tris-glycine, 150mM sodium chloride, 0.05% sodium azide.
Specificity:Recognizes the human SSAT-1 at ~27kD. Species crossreactivity: mouse, rat, porcine, hamster, chicken, bovine and xenopus.
Isotype:IgG
Calc Applications Abbrev:WB
Calc Crossreactivity:Bo Ch Hm Hu Mo Po Rt Xe
Immunogen:Synthetic peptide corresponding to the C-terminal region within aa100-171 of the human SSAT-1 (Swiss-Prot# P21673). Species sequence homology: zebrafish, 86%.