Autophagy is an important mechanism for nonselective intracellular breakdown whereby cytosol and organelles are encapsulated in vesicles, which are then engulfed and digested by lytic vacuoles/lysosomes.
ATG5 is required for autophagy.
It conjugates to ATG12 and associates with isolation membrane to form autophagosomes.
The conjugate detaches from the membrane immediately before or after autophagosome formation is completed.
Conjugation to ATG12 is essential for autophagy, but is not required for association with isolation membrane.
ATG5 also plays an important role in the apoptotic process.
Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications have not been tested.
Recommended Dilution:Western Blot: 1:500Immunohistochemistry: 2.5ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:Mouse wildtype ES cell lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.09% sodium azide, 30% glycerol.
Specificity：Recognizes ATG5. Selective for the full-length and calpain cleaved isoform proteins. The short isoform is missing aa1-79. The calpain cleaved form of ATG5 is missing aa195-275.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Mo
Immunogen：Synthetic peptide corresponding to an N-terminal region (within residues 1-50) of the human ATG5 protein. (Swiss-Prot# Q9H1Y0). Species sequence homology: human, rat, bovine, porcine, monkey and Xenopus, 100%; zebrafish, 94%
