cAMP responsive element binding protein (CREB) is a 43kD leucine zipper transcription factor that stimulates transcription and gene expression after its phosphorylation on serine residues.
CREB plays an important role in development, glucose homeostasis and growth-factor-dependent cell survival and is implicated in learning and memory.
The phosphorylation of CREB at GSK-3β and PKA sites (serines 129 and 133, respectively) is essential for its transcriptional activity.
Applications:Suitable for use in ELISA, Western Blotting.
Other applications not tested.
Recommended Dilution:Western Blotting: 1:1000-1:2000Optimal dilutions to be determined by the researcher.
Positive Control Used: NIH3T3 cells +/- PDGF, Y-1 cells.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified from rabbit serum by sequential epitope-specific chromatography. Negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated CREB. The final product is generated by affinity chromatography using a CREB-derived peptide that is phosphorylated at serines 129 and 133.
Form：Supplied as a liquid in PBS (without Mg2+ and Ca2+), 1mg/ml BSA (IgG, protease free), pH 7.2, 0.09% sodium azide, 50% glycerol.
Specificity：Reactivity: Human and mouse CREB when phosphorylated at Ser129 and Ser133. Species sequence homology: Rat (100%).
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic phosphopeptide derived from a region of human CREB that contains serines 129 and 133. The sequence is conserved in mouse and rat.
