Human Wnt-6 is a 46 kD, secreted, glycoprotein of the Wnt family.
Human Wnt-6 is synthesized as a 365 amino acid (aa) precursor that contains a 341 aa mature region.
The mature molecule contains 24 cysteines and one potential N-linked glycosylation site.
Wnt-6 is produced by mammary fibroblasts and ductal epithelium, intestinal crypt cells and limb bud ectoderm.
Over the regions used as immunogen, the aa sequences of human, mouse and canine Wnt-6 are identical.
Applications:Suitable for use in Western Blot, Immunohistochemistry and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1-0.2ug/mlImmunohistochemistry: 5-15ug/mlELISA: 0.5-1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by human Wnt-6 affinity chromatography.
Form：Supplied as a lyophilized powder from a 0.2um filtered solution in PBS, 5% trehalose. Reconstitute with 1ml sterile, ddH2O or sterile 40-50% glycerol.
Specificity：Recognizes human Wnt-6.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu
Immunogen：E. coli-derived recombinant human Wnt-6 peptide containing rhWnt-6 (aa35-75 and 238-299) joined by a linker peptide, UniProt # Q9Y6F9
