Mouse Wnt-2b (also Wnt-13) is a 389 amino acid, 42kD member of the large and highly conserved Wnt family of secreted, cysteine-rich glycoprotein signaling molecules.
Wnt proteins are associated with developmental and carcinogenic processes.
Wnt-2b may be necessary to maintain stem cells in an undifferentiated state.
As a type II transmembrane protein with a long C-terminal extracellular and a short N-terminal cytoplasmic domain, Wnt-2b is presumably cleaved within its extracellular region to release the secreted form.
In the region from aa 64-104, mouse Wnt-2b is 100% identical to the human and rat protein, and over the 251-312 aa region also used as immunogen, mouse Wnt-2b is 97% and 100% identical to the corresponding human and rat sequences, respectively.
Applications:Western Blot: This antibody can be used at 0.1-0.2ug/ml with the appropriate secondary reagents to detect Wnt-2b.
The detection limit for rmWnt-2b is approximately 5 ng/lane and 1 ng/lane under non-reducing and reducing conditions, respectively.
In this format, this antibody shows approximately 20% cross-reactivity with rhWnt-2, and less than 5% cross-reactivity with rhWnt-7a, -9a, rmWnt-1, -3a, -4, -5a, -5b, -8a, -8b, -9b, -10a, -10b, -11, -16
Immunocytochemistry: This antibody has been used at a concentration of 10ug/ml to detect Wnt-2b in BT20 human breast carcinoma cells.
Cells were fixed with PBS containing 4% paraformaldehyde for 20 minutes at room temperature and blocked with PBS containing 10% normal donkey serum, 0.1% Triton X-100, and 1% BSA for 45 minutes at room temperature.
After blocking, cells were incubated with diluted primary antibody overnight at 4° C followed by NorthernLightsTM-557-coupled anti-goat IgG at room temperature in the dark for one hour.
Between each step, cells were washed with PBS containing BSA.
Direct ELISA: This antibody can be used at 0.5-1.0ug/ml with the appropriate secondary reagents to detect mouse Wnt-2b.
The detection limit for rmWnt-2b is approximately 0.5 ng/well.
Optimal dilutions should be determined by each laboratory for each application.
Reconstitution:Reconstitute with sterile PBS.
If 0.5 mL of PBS is used, the antibody concentration will be 0.2 mg/mL.
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by mouse Wnt-2b affinity chromatography.
Form：Supplied as a lyophilized powder from a 0.2um filtered solution in PBS, 5% trehalose.
Specificity：Selected for its ability to recognize mouse and human Wnt-2b in the applications listed.
