N-WASP is a ubiquitously expressed member of the WASP family of proteins.
The 65 kD, 505 amino acid, N-WASP seems to play a central role as a node interconnecting various actin networks.
Over the region used as immunogen, human N-WASP is greater than 98% identical to the corresponding mouse and canine protein sequences.
Applications:Western Blot: This antibody can be used at 1.0ug/ml with the appropriate secondary reagents to detect N-WASP
Immunocytochemistry: This antibody has been used at a concentration of 10ug/ml to detect human N-WASP on A431 human epidermoid carcinoma cells stimulated with EGF.
Cells were fixed with PBS containing 4% paraformaldehyde and blocked with PBS containing 10% normal donkey serum, 0.1% Triton X-100, and 1% BSA.
After blocking, cells were incubated with diluted primary antibody followed by Rhodamine RedTM-coupled anti-goat IgG at room temperature in the dark.
Between each step, cells were washed with PBS containing BSA.
Direct ELISA: This antibody can be used at 0.5-1.0ug/ml with the appropriate secondary reagents to detect human N-WASP.
The detection limit for rhN-WASP is approximately 1.0 ng/well.
Optimal dilutions should be determined by each laboratory for each application.
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
Reconstitute to nominal volume by adding sterile PBS with 40-50% glycerol and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by human N-WASP affinity chromatography.
Form：Supplied as a lyophilized powder from a 0.2um filtered solution in PBS with 5% trehalose. Reconstitute with sterile PBS, 40-50% glycerol. If 0.5ml is used, the concentration will be 0.2mg/ml.
Specificity：Selected for its ability to recognize human N-WASP in the applications listed.
