VEGF (Vascular Endothelial Growth Factor) is a homodimeric, disulfide-linked glycoprotein involved in angiogenesis which promotes tumor progression and metastasis.
It exhibits potent mitogenic and permeablility inducing properties specific for the vascular endothelium.
Multiple isoforms of VEGF containing 206-, 189-, 165-, and 121-amino acid residues have been described which arise from alternative splicing of a single gene.
The smaller two isoforms, VEGF 165 and VEGF121, are secreted proteins and act as diffusible agents, whereas the larger two remain cell-associated.
VEGF/VPF plays an important role in angiogenesis, which promotes tumor progression and metastasis.
Applications:Suitable for use in Western Blot, Immunocytochemistry and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1-0.2ug/mlImmunocytochemistry: 5-15ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
Reconstitute to nominal volume by adding sterile TBS with 40-50% glycerol and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：50ug
Host：goat
Source Antibody：canine
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from a 0.2um filtered solution in PBS. Reconstitute with sterile TBS, pH 7.3 (20mM Trizma base, 150mM sodium chloride) containing 0.1% BSA, 40-50% glycerol. If 1ml of buffer is used, the concentration will be 50ug/ml.
