UTF1 (undifferentiated embryonic cell transcription factor 1) is a 47-57kD transcriptional coactivator.
It is expressed mainly in embryonic stem cells, is regulated by Oct-3/4, and promotes rapid cell proliferation.
Human UTF1 is a 341aa phosphoprotein.
It is rich in Proline and Arginine residues and contains a C-terminal Leu-zipper (aa286-313) that is known to interact with the metal-binding motif of ATF-2.
Unlike mouse UTF1 which contains an alternate start site at Met 43, human UTF1 lacks this Methionine and shows only one isoform.
Over aa44-153, human UTF1 shares 81% aa sequence identity with mouse UTF1.
Applications:Suitable for use in Western Blot, Immunocytochemistry and ELISA.
Other applications have not been tested.
Recommended Dilutions:Western Blot: 0.1ug/ml using Recombinant Human UTF1
Immunocytochemistry: 5-15ug/ml using Immersion fixed D3 mouse embryonic stem cell line and human BG0IV stem cells
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Specificity：Recognizes human UTF1.
Isotype：IgG
Calc Applications Abbrev：E IC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Ser44-Lys153 of human UTF1 expressed in E. coli. Species Sequence Homology: mouse.
