84-1090-30　［取扱停止］Tyrosine Hydroxylase, phosphorylated (Ser31) (TH, TYH, Tyrosine 3 Hydroxylase, Tyrosine 3 Monooxygenase) 100ul　T9237-05J

特徴

• Rat tyrosine hydroxylase (TH) is a pterin-dependent monooxygenase that catalyzes the hydroxylation of tyrosine to DOPA through the use of a non-heme iron.
• It is a 55 kD, 498 amino acid (aa) α-helical protein that runs anomalously at 62 kD in SDS-PAGE.
• The molecule contains a 164 aa N-terminal regulatory region and a 334 aa C-terminal catalytic domain.
• Its activity is regulated at the post-transcriptional level by phosphorylation of serine and feedback by catecholamines.
• Four serines are known to be phosphorylated at aa positions 8, 19, 31, and 40.
• Different kinases contribute to different phosphorylation patterns.
• For example, MAPKAPK-2 and CaMKII act on S19 and S40, PKA phosphorylates only at S40, while Cdk5 phosphorylates S31.
• Variable site phosphorylations have variable effects.
• S40 phosphorylation blocks catecholamine feedback inhibition.
• Subsequent phosphorylations at S19 or S31 likely stabilize an otherwise unstable enzyme phosphorylated only at S40, contributing to increased enzyme activity.
• Phosphorylation at S8 is likely to be physiologically unimportant.
• Applications:Western Blot: 1:1000Immunofluorescence: 1:1000 (frozen sections)Immunohistochemistry: 1:1000 (frozen sections) Optimal dilutions should be determined by each laboratory for each application.
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage, store at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.