Applications:Suitable for use in Western Blot and Direct ELISA.
Other applications have not been tested.
Recommended Dilutions:Western Blot: This antibody can be used at 0.1-0.2ug/ml with the appropriate secondary reagents to detect mouse Trypsin 3.
The detection limit for rmTrypsin 3 is approximately 5 ng/lane under non-reducing and reducing conditions.
In this format, this antibody shows approximately 20% cross-reactivity with rhTrypsin 1, rhTrypsin 2, and rhTrypsin 3
Immunoprecipitation: This antibody has been used to immunoprecipitate rmTrypsin 3 from conditioned media of transfected NS0 cells.
Direct ELISA: This antibody can be used at 0.5-1.0ug/ml with the appropriate secondary reagents to detect mouse Trypsin 3.
The detection limit for rmTrypsin 3 is approximately 3 ng/well.
Optimal dilutions should be determined by each laboratory for each application.
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
Reconstitute to nominal volume by adding sterile PBS with 40-50% glycerol and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by mouse Trypsin 3 affinity chromatography.
Form：Supplied as a lyophilized powder from a 0.2um filtered solution in PBS with 5% trehalose. Reconstitute with sterile PBS, 40-50% glycerol. If 0.5ml is used, the concentration will be 0.2mg/ml.
Specificity：Selected for its ability to recognize mouse Trypsin 3 in direct ELISAs and Western Blots.
